Survivability of caprine oocytes vitrified in conventional and open pulled straws
نویسندگان
چکیده
منابع مشابه
Open pulled straws for vitrification of mature mouse oocytes preserve patterns of meiotic spindles and chromosomes better than conventional straws.
Vitrification of oocytes has been applied recently for humans, but remains elusive. The microtubules of oocytes are vulnerable to cryoprotectants and thermal changes. Using mouse oocytes, the effects of vitrification in open pulled straws (OPS) were investigated on survival, the meiotic spindle, and chromosomes and compared with conventional straws. Mature oocytes were allocated to four groups ...
متن کاملAseptic technology of vitrification of human pronuclear oocytes using open-pulled straws.
BACKGROUND The aim of this study was to compare the viability of human pronuclear oocytes subjected to vitrification using cooling by direct submerging of open-pulled straws in liquid nitrogen versus vitrification by cooling of open-pulled straws located inside a closed 0.5 ml straw (aseptic system). METHODS Two- and three-pronuclei stage oocytes (n=114) were cryopreserved in super-open-pulle...
متن کاملThe Survival Rate, Fertilization and Developmental Capacity of Mature Mouse Oocyte Which Vitrified in Open Pulled Straw
Purpose: The aim of this study was to evaluate the effect of vitrification in open pulled straw on survival rate, fertilization and development capacity and comparison with conventional straw. Materials and Methods: NMRI mice were used for retrieval of oocytes. After administration of HMG and HCG, the mice scarified by cervical dislocation. Mice's abdomens were dissected and mature oocytes wer...
متن کاملComparison of The Effects of Vitrification on Gene Expression of Mature Mouse Oocytes Using Cryotop and Open Pulled Straw
Objective Oocyte cryopreservation is an essential part of assisted reproductive technology (ART) which was recently introduced into clinical practice. This study aimed to evaluate the effects of two vitrification systems (Cryotop and Open Pulled Straw, OPS) on mature oocytes gene expressions. MaterialsAndMethods The survival rate of metaphase II (MII) mouse oocytes were assessed after cryoprese...
متن کاملVitrification of human oocyte using cryoloop
Background: The cryopreservation of human oocyte would make a significant contribution to infertility treatment, such as using it for oocyte donation and for patients a bout to lose ovarian function due to surgery or chemotherapy. Despite of using standard freezing straws and cryovials or even open pulled straws, only a few successful pregnancies have been arisen from cryopreserved human oocyte...
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ژورنال
عنوان ژورنال: TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES
سال: 2014
ISSN: 1300-0128,1303-6181
DOI: 10.3906/vet-1404-43